Type III Protein Translocase HrcN IS A PERIPHERAL MEMBRANE ATPASE THAT IS ACTIVATED BY OLIGOMERIZATION*
نویسندگان
چکیده
From the Institute of Molecular Biology and Biotechnology, FORTH and Department of Biology, University of Crete, P.O. Box 1527, GR-711 10 Iraklio, Crete, Greece, §M.E. Mueller Institute for Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland, the ¶Department of Biological Sciences, Imperial College at Wye, University of London, Ashford, Kent, United Kingdom TN25 5AH, the Laboratory of Biological Chemistry, Medical School, University of Ioaninna, Ioannina 45110 Greece, and the **Molecular Genetics Unit CNRS FRE 2364, Institut Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France
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Double hexameric ring assembly of the type III protein translocase ATPase HrcN.
The specialized type III secretion (T3S) apparatus of pathogenic and symbiotic Gram-negative bacteria comprises a complex transmembrane organelle and an ATPase homologous to the F1-ATPase beta subunit. The T3S ATPase HrcN of Pseudomonas syringae associates with the inner membrane, and its ATP hydrolytic activity is stimulated by dodecamerization. The structure of dodecameric HrcN (HrcN12) deter...
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